social memory mice

Latency to immobility and immobility time (none of the four paws moving) were measured during a 6 min trial. in social memory. Nesting behavior, group sleeping, physical appearance, and absence of hind limb clasping were similar in both genotypes. doi: 10.1080/09513590.2016.1197197, Zhang, Z., Chu, S. F., Mou, Z., Gao, Y., Wang, Z. Data showed that both WT and STEP KO mice presented similar levels of activity in the more aversive areas, the open arms of the plus maze and the light compartment of the dark-light box. Genes Brain Behav. Time exploring three different scents including clean bedding and two different social odors (social A and social B) in the olfactory habituation/dishabituation test. Vasopressin 1b receptor knockout impairs memory for temporal order. The PVN is known to integrate information to orchestrate stress responses. Genome Res. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. We showed that anisomycin impaired the consolidation of social fear memories in a time-point-dependent manner. Behav. LED optic fibers implanted in the CA2 were used to stimulate the circuit. Office of Communications and Public Liaison. To address this possibility, WT and STEP KO mice were subjected to the olfactory habituation/dishabituation test. However, we observed significantly increased basal extracellular concentrations of DA, and higher DA output in response to inhibition of DA reuptake in the dorsal striatum of STEP KO mice, without alterations in the total levels of D1R and D2R. Subjects were first habituated to the empty apparatus, a three-chamber box consisting of three interconnected lined compartments (DeVito et al., 2009) with open doors, for 10 min trial/day, for 3 days. (A) Representative photomicrograph illustrating the location (arrows) of the dialysis probe after staining with neutral red. Table 1. Alline C Campos 1 ,2 , Franciele F Scarante 1 , Sabine Martin 2,3 , Marcio Lazzarini 2 , Doris STEP KO mice have normal olfactory function. Habituation was defined as the progressive decrease in olfactory investigation towards a repeated presentation of the same odor stimulus. doi: 10.1523/JNEUROSCI.15-02-01532.1995, Can, A., Dao, D. T., Terrillion, C. E., Piantadosi, S. C., Bhat, S., and Gould, T. D. (2012). At a Glance. Cell 90, 895–905. Now an NIMH-funded study on how mice form social memories gives new meaning to that timeless phrase. Mol Psychiatry. Lesions to the CA2 region of the hippocampus impair social memory in mice. 3f, Fig. “Genotype” effect n.s., ***p < 0.001 “object” effect (two-way ANOVA). However, social memory and social behaviors have been barely studied in this mouse model, and there are also reports showing that alterations in the ERK pathway may underlie altered social behavior (Satoh et al., 2011; Faridar et al., 2014). Results are presented as mean ± SEM [n = 11–12 for (A,B); n = 9–10 for (C,D)]. *p < 0.05 “genotype” effect (Student’s independent t-test). 67, 433–447. Thus, our findings suggest that STEP KO mice present decreased motivation to cope with new environmental or social stimuli. Comparison between the first and the third trial also showed differences between genotypes (ANOVA, WT: 9.95 ± 17.33 s, STEP KO: 65.99 ± 11.73 s, F(1,18) = 6.85, p < 0.05) in the dishabituation, thus indicating social memory alterations. STEP is highly expressed in the striatum and at lower levels in the cortex, hippocampus, amygdala and other brain regions, except in the cerebellum (Lombroso et al., 1991, 1993; Boulanger et al., 1995). Mice are first presented with three non social odor cues. In that study, only a direct interaction test was performed, a smaller group of animals was tested, and data of social memory assessment was not provided (Venkitaramani et al., 2011). Mol. doi: 10.1007/s00213-008-1205-9, Castonguay, D., Dufort-Gervais, J., Ménard, C., Chatterjee, M., Quirion, R., Bontempi, B., et al. Genetic manipulation of STEP reverses behavioral abnormalities in a fragile X syndrome mouse model. 287, 20942–20956. a, Social memory by Oxt–/– (open symbols) and Oxt+/+ (filled symbols) male mice was measured as a difference in olfac-tory investigation. Modulation of the glutamatergic transmission by Dopamine: a focus on Parkinson, Huntington and Addiction diseases. Behav. Importantly, while WT mice displayed social memory and spent more time exploring the “stranger” mouse than the “known” mouse cage (Student’s t-test, t(1,22) = 4.46, p < 0.001), STEP KO mice showed no preference for any mouse cage (Student’s t-test, t(1,20) = 0.37, n.s.) Psychopharmacology 163, 434–458. Ganglioside GQ1b induces dopamine release through the activation of Pyk2. Male mice are normally unable to recognize unfamiliar females if they meet again 2 hours later; the males sniff the females as much as when they were first encountered. Social memory was also assessed in the 11-trial habituation/dishabituation social recognition test. Synapse 63, 69–81. Basal dopamine (DA) release in the dorsal striatum is increased in STEP KO mice. Time of interaction was measured during a 5 min trial. 64, 65–87. (2012). This drastically increased the staying power of the male’s memory for the female. Chemically blocking vasopressin activity in the CA2 prevented this social memory enhancement. Psychiatry 2, 271–281. The subject mouse was left for 5 min in one of the spaces to adapt to the barrier. 148, 185–198. J. Neurosci. doi: 10.1523/JNEUROSCI.20-24-09320.2000, Venkitaramani, D. V., Paul, S., Zhang, Y., Kurup, P. K., Ding, L., Tressler, L., et al. International Journal of Molecular Sciences Communication Social Fear Memory Requires Two Stages of Protein Synthesis in Mice Johannes Kornhuber 1 and Iulia Zoicas 1,2,* 1 Department of Psychiatry and Psychotherapy, Friedrich-Alexander University Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany; Johannes.Kornhuber@uk-erlangen.de Upregulation of CREB-mediated transcription enhances both short- and long-term memory. Figure 5. doi: 10.1073/pnas.1013543107, Zhang, Z., Zhang, Y., Mou, Z., Chu, S., Chen, X., He, W., et al. Actually, STEP KO mice showed diminished latency to enter the open arms of the plus maze compared to the control group (Table 1), thus indicating that they do not present altered anxiety levels. STriatal-Enriched protein tyrosine Phosphatase (STEP) is a neural-specific phosphatase that opposes the development of synaptic strengthening through the regulation of multiple kinases and glutamate receptor subunits critical for synaptic plasticity. 67:e4389. Given that STEP KO mice have intact motor capabilities (present results) and motor coordination (Venkitaramani et al., 2011; Sukoff Rizzo et al., 2014), this discrepancy might be explained by differences in methodological procedures. Proc. 12:e1001923. J. Huntingtons Dis. doi: 10.1111/j.1601-183x.2005.00123.x, Stevenson, E. L., and Caldwell, H. K. (2014). Genome 8, 711–713. Mice were used in multiple (six) behavioral tests with the exception of the juveniles used in long‐term social recognition memory test (Section Long‐term social recognition). Among the different laboratory tasks used to investigate recognition memory in rats and mice, the social recognition/social discrimination task has several advantages. Fgf9 Y162C Mutation Alters Information Processing and Social Memory in Mice. Front. 89, 1–9. doi: 10.1111/j.1601-183x.2012.00775.x, Spencer, C. M., Alekseyenko, O., Serysheva, E., Yuva-Paylor, L. A., and Paylor, R. (2005). Since mice with a genetic deletion of STEP displayed social memory impairment that could not be explained by the presence of olfactory defects, alterations in sensorimotor functions or increased anxiety levels, we next sought to analyze whether social patterns were also affected. A mouse juvenile (C57BL/6J, 15–35 days old) was introduced in the subject’s cage during a 4 min trial for the sampling phase. [Epub ahead of print]. Our data indicate that OT is necessary for the normal development of social memory in mice and support the hypothesis that social memory has a neural basis distinct from other forms of memory. Epub 2009 Jun 13. Neuropharmacology 128, 43–53. This experiment measures olfaction, memory, and social interest. Training phase. ; “object” effect, F(1,20)= 48.76, p < 0.001) and there were no significant differences in time of exploration of both objects (Student’s t-test WT: 30.35 ± 5.74 s and STEP KO: 16.72 ± 4.99 s, t(1,21) = 1.17, n.s. doi: 10.1111/j.1601-183x.2012.00781.x, Hitti, F. L., and Siegelbaum, S. A. doi: 10.1016/j.neuroscience.2012.07.069, Pagani, J. H., Zhao, M., Cui, Z., Williams Avram, S. K., Caruana, D. A., Dudek, S. M., et al. Here we describe in detail three protocols to quantitatively measure sociability, one of the most frequently investigated social behavior phenotypes in mice, using a three‐chamber sociability test. We thankDr. (2014). Eur. The use of behavioral test batteries: effects of training history. (2012). Cell. The synapsins: Key actors of synapse function and plasticity. Subject mice (transgenic mice and their wildtype littermates) should be group housed (2-5 mice) for best results as single housing mouse can lead to decreased social recognition memory (Kogan et al., 2000). Behav. (2002). The tyrosine phosphatase STEP is involved in age-related memory decline. doi: 10.1515/JBCPP.1991.2.4.315, Rodriguiz, R. M., Chu, R., Caron, M. G., and Wetsel, W. C. (2004). doi: 10.1523/JNEUROSCI.5488-08.2009, Engelmann, M., Hädicke, J., and Noack, J. The researchers first traced the neurons to confirm a single, anatomically distinct circuit from the PVN that releases vasopressin in the CA2. (1997). Nat. Front. Two-way ANOVA showed a significant effect of time (F(18,306) = 24.97; p < 0.001), a quasi-significant effect of genotype (F(1,17) = 4.254; p = 0.054) and a significant time × genotype interaction (F(18,306) = 2.929; p < 0.001, Figure 5D), pointing at an increased release of DA in the STEP KO mice compared to the WT group. 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